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Blocking buffer: 10 mM Tris-HCI pH , mM NaCI, 5 mg/ml Torula yeast core RNA" (Sigma) • NWB buffer: 10 mM Tris-HCI pH , 1 mM EDTA, 50 mM NaCI, % Ficoll, % polyvinyl pyrrolidone, % Then wash the filters once in 20 ml of renaturation buffer at room temperature for 10 min with mild shaking. 4. Good price, as described s 2 ( Good price. t a ( b n ( 1 2 ( l 5 ( d 4 () ). | eBay!. 1 2 ( good price, as described s 2 ( Good price. t a ( b n ( l 5 ( d 4 () ). | eBay!.